Coding

Part:BBa_K209016:Design

Designed by: Ryan Quan   Group: iGEM09_UCSF   (2009-09-11)


Aar1 C-D part, PIPK1 gamma (Dictyostelium discoideum)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 592
    Illegal PstI site found at 904
    Illegal PstI site found at 1198
    Illegal PstI site found at 1708
    Illegal PstI site found at 1808
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 592
    Illegal PstI site found at 904
    Illegal PstI site found at 1198
    Illegal PstI site found at 1708
    Illegal PstI site found at 1808
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 592
    Illegal PstI site found at 904
    Illegal PstI site found at 1198
    Illegal PstI site found at 1708
    Illegal PstI site found at 1808
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 592
    Illegal PstI site found at 904
    Illegal PstI site found at 1198
    Illegal PstI site found at 1708
    Illegal PstI site found at 1808
    Illegal NgoMIV site found at 787
    Illegal NgoMIV site found at 1508
    Illegal NgoMIV site found at 1676
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 807


Design Notes

Aar1 CD part in pDONR221. BBF RFC 28.


Source

from plasmid

References